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Molecular and subcellular localization of the potassium voltage-gated channel, Kv3.4, and a novel membrane-associated potassium channel, Kv2.4, in rat peripheral autonomic ganglia.
The characteristics of K+ channels in neurons of sympathetic ganglia are distinct from those in most other CNS neurons. Of the types of K+ channels that are found in neurons of sympathetic ganglia, the large-conductance Ca2+- and voltage-activated K+ channel (BK) and Ca2+-independent K+ channels that have more negative membrane potentials than the Ca2+-activated K+ channel (IK) are present in sympathetic neurons. A novel member of the voltage-activated K+ channel superfamily has recently been cloned, and the genomic sequence predicts that this channel will be localized to plasma membranes. Here, we report the cellular localization of the three main types of voltage-activated K+ channels (Kv1.4, Kv2.1, and Kv3.1) and of a novel K+ channel, Kv2.4, within sympathetic neurons. We demonstrated that both Kv1.4 and Kv3.4 proteins, the members of the Kv1 and Kv3 subfamilies, were primarily localized to the cell bodies and dendrites of sympathetic neurons, while Kv2.4 protein was localized to the plasma membranes in these neurons. These findings
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